Schwaggy P's Random Stuff

Armdog

Master Grower
Materials:
Silver Nitrate (AgNO3)
Sodium Thiosulfate or Sodium Thiosulfate Pentahydrate (Na2S2O3 or Na2S2O3-5H2O)
Distilled water
Gloves
Mask
Containers
Spray bottle
Volume measuring device
Scale

We will be preparing 2 separate solutions that will be mixed later for spraying.
❗Labeling your containers with A and B before will help to keep them from being confused.
❗Wear the gloves throughout the preparation and application of STS, and wear the mask during the spray application of STS.


SOLUTION A – Silver Nitrate
1) Mass 1.7g of Silver Nitrate, and add this to your container labeled A (pic 1,2)

❗Be careful when handling the Silver Nitrate as it can stain your skin.

2) Add 100mL of distilled water to container A (pic 3).

3) Agitate the mixture until the Silver Nitrate dissolves.

❗Do not use metal objects to stir this solution (you can use plastic spoons, straws, or glass)

4) Store Solution A in a dark bottle or in a bag, and keep it in a dark place until needed for mixing STS spray solution (pic 4).

SOLUTION B – Sodium Thiosulfate
‼TAKE NOTE OF WHICH SODIUM THIOSULFATE COMPOUND YOU HAVE: EITHER SODIUM THIOSULFATE OR SODIUM THIOSULFATE PENTAHYDRATE! The only difference between the two is that one has water in the compound that can throw off the concentrations of our target solution if no tweak to the mass is made.

5) Mass either: 6.32g Sodium Thiosulfate OR 9.92g Sodium Thiosulfate Pentahydrate and add this to your container labeled B (pic 5,6).

6) Add 400mL of distilled water to container B (pic 7).

7) Agitate the mixture until the Sodium Thiosulfate dissolves.

❗This mixture does not need to be protected from light (pic 8). Both mixtures can be stored for long periods of time and mixed when needed for reversal.

Preparation of STS
We will be mixing an amount of Solution A and Solution B to create the Silver Thiosulfate. It is very important to note:
‼WE MUST ADD SOLUTION-A TO SOLUTION-B WHEN MIXING.

To create a stock solution of STS, we will need a ratio of [1partA : 4partsB]. You can tailor your volume to make only what you will need for your spraying application.

For the purposes of this example, I will prepare a stock solution of specific volume:

8) Add 5mL of Solution A to 20mL of Solution B. You now have a stock solution of Silver Thiosulfate (STS) (pic 9).

❗The STS solution is not very stable, so it is best to only prepare what you will be using for one round of spraying.

Dilution of STS for Spray Application

Depending on the particular plant you would like to reverse, you may need stronger/weaker STS spray. You can use 1:2, 1:3, 1:4, etc. ratios of STS:Water.

I will be using a 1:2 ratio and make any adjustments based on how the plant is responding.

9) Since we have 25mL of STS prepared, we can add 50mL of distilled water (pic 10). We now have 1:2 STS reversal spray solution. Add this mixture to your spray bottle if you mixed in a different container (pic 11).

Application of STS
❗You could spray a target female while in the growing space around other plants (assuming you get no spray on any other plants) with success, but we will remove the target female and segregate her for spraying.

10) Place a drop cloth, cardboard, or cover under the female to keep overspray contained. This also has the effect of shielding the soil from STS overspray (pic 12).

11) Spray the female liberally, either as with any foliar spray application or targeting only nodes. I have chosen to spray the entire plant (pic 13,14).

12) Allow the female to dry before returning her to the growing space.

You will repeat the STS spray application every 7-10 days until you observe the development of male parts. This means there will only need to be 2-3 applications of the STS spray for successful reversal and collection of pollen.

❗The female will take about 2 weeks to show the fruits of reversal. Some suggest to begin the spraying 2 weeks in advance of flower to keep her closer to a schedule seen with males. For this plant, I will be spraying at the start of flip.

Here is the Chemdog’91(skva) progressing through the reversal process, culminating in harvestable pollen around day 29.
REVERSED POLLEN COLLECTION
There are many ways to go about harvest and collection of fem pollen, but this is how I go about the process.

Materials:
Reversed pollen sacs
Grinder screen and lid (no kief catch)
Scraper (cut from plant tag)
Pollen container
Razor
Work Surface (black paper plate)

1) You can better prep your pollen by separating the “bananas” from any other plant matter, but I left them on in this picture (pic 1).

❗Allowing your freshly plucked pollen sacs a day to dry before attempting this will aid in pollen collection.

2) Place the bananas in the grinder screen (no kief catch attached to bottom), and gently agitate/chop with the scraper (pic 2).

3) Holding the screen with one hand, gently tap the side with the grinder lid. You will see the bananas skid across the screen (pic 3).

4) Give the screen a minute before gently lifting to reveal the separated pollen (pic 4).

5) Scrape the pollen with your razor and store into your pollen container.

With your fem pollen created and collected, you can now pollinate your females to create feminized seeds. Feel free to marry this with the single branch pollination process to create small batch fem seeds for yourself without sacrificing whole plants.
Thanks for the tutorial
 
I'm very pleased with the Skunky D. When I started the Chem-Skunk project with the different Chemdogs, I wanted plants that had the offensive profile in the nose with a slightly more compact "Skunk" look and Chemdog chemotypes. The Chem D is really fitting the bill and in F1 no less. The first pheno pictured has a "jagged" overall look to her that reminds me of some Mass Super Skunk crosses I've grown. The Skunky Brewster is allowing a lot of the Chem D terp profile to express throughout the population with a more compact stature. I have yet to spot any signs of hermaphroditism in the Skunky D. I have pollinated for a next gen, but I'm sure most would be pleased with what can be found from a pack of the F1.
skunkyd1.jpg

Different pheno, just as offensive on the nose with a more rounded look. The Skunky Brewster has increased the frost production and shortened the overall height (not stumpy, just not as lanky). The stem strength has also been improved from the Chem D; I have been able to avoid tying up individual branches. If these ladies can finish in the 9-10 week window and have that Chem D kick, we may have something here. 😬🤞
skunkyd-2.jpg


skunkyd side nug.jpg
 

Phylex

Grow God
Very nicely done. I always dig your detailed write ups with your explanations and findings regarding the current project and your laid out expectations. I know you mentioned taking them further, but congrats on finding most of what you're looking for in the F1's. Sounds like you picked a great pairing!
 

Texagonian

Outlaw
@Schwaggy P , I have a question about storing harvested pollen. I have been in the process of harvesting some from a very nice male plant I decided to keep for the purpose of doing my first chuck. That being said, I have the collected pollen in a sealed glass vessel and is in a dark cabinet with no elements such as light, air, or moisture that can invade. Do you feel this is acceptable or should I find other alternatives? Thank you in advance for any input ✌🏻 Also, I plan on using the tent I’m growing the male plant in currently for other grows, what is a good way to make sure there aren’t any accidental pollinations? I planned on doing the usual spray and wipe down to reset the area but that pollen is everywhere in that tent. Thanks again!
 
I would just like to buy untested Schwaggy beans I do not want to wait LOL I am getting old
:LOL: Shouldn't be much longer. I've decided to put out steps of larger projects in progress, so it's not a multi-year wait for something in particular. For example, I have a preliminary drop in mind that includes Hell's Angels OG BX1 (which will be backcrossed a few more times to really cement the H.A.OG expressions in reg seed form). I'm currently coordinating with the seed bank on the specific details and will make a formal announcement post with the drop date and content. Thanks for the interest! 😁
 
I have the collected pollen in a sealed glass vessel and is in a dark cabinet with no elements such as light, air, or moisture that can invade. Do you feel this is acceptable or should I find other alternatives?
This would be an okay way to store the pollen if you're planning on using it in the near future (1-2weeks). For longer term storage, I would suggest keeping the airtight pollen container in the freezer. This should keep your pollen viable for about a year before you begin to notice a fall off in viability.

pollen.jpg
After pollen collection, depending on the amount of pollen in the vial, I like to let the vial sit without the cap for a 1-2 days to fully dry before storage (1). Then I cap the vial, and put it in a small 4mil baggie with a desiccant (2). The vials have a drawer in the freezer to stay until I decide to make a withdrawal (3).
 

Baja.Beaches

Master Grower
:LOL: Shouldn't be much longer. I've decided to put out steps of larger projects in progress, so it's not a multi-year wait for something in particular. For example, I have a preliminary drop in mind that includes Hell's Angels OG BX1 (which will be backcrossed a few more times to really cement the H.A.OG expressions in reg seed form). I'm currently coordinating with the seed bank on the specific details and will make a formal announcement post with the drop date and content. Thanks for the interest! 😁
So you are finally going public? Congratulations, both to you & the community that will get some killer genetics.

Have you reached an agreement with a seed bank?

Jabba’s Cure update. Dang that outdoor one is pretty. 2nd is the last look at the indoor now hanging in my garage, I will be smoking that in a few days. Sure is stinky.

Keep us posted on your coming out party!

DSC03922.jpg



DSC03912.jpg
 
This would be an okay way to store the pollen if you're planning on using it in the near future (1-2weeks). For longer term storage, I would suggest keeping the airtight pollen container in the freezer. This should keep your pollen viable for about a year before you begin to notice a fall off in viability.

After pollen collection, depending on the amount of pollen in the vial, I like to let the vial sit without the cap for a 1-2 days to fully dry before storage (1). Then I cap the vial, and put it in a small 4mil baggie with a desiccant (2). The vials have a drawer in the freezer to stay until I decide to make a withdrawal (3).
Schwaggy, when you pull the vials to be used from the freezer, do you go immediately to the plants and use it right away? Or do you let it sit and thaw for a while? I collected and stored some pollen from a male a few months back. After following all of the above steps, and sealing them up, I've tried pollinating a number of different plants with that pollen and have had no success with it. Tried it thawed on a few plants. Tried it right out of the freezer on a different batch of plants. Had a buddy try some on a couple of his plants, still nothing. Is it possibly just a sterile male that was collected from? Is that fairly common? Thanks in advance.

Count me in, as an interested party in your Schwaggy beans. Really like seeing what you are doing. Wishing you all the best.
 
So you are finally going public? Congratulations, both to you & the community that will get some killer genetics.

Have you reached an agreement with a seed bank?

Jabba’s Cure update. Dang that outdoor one is pretty. 2nd is the last look at the indoor now hanging in my garage, I will be smoking that in a few days. Sure is stinky.

Keep us posted on your coming out party!
Thank you! I have the okay from a bank to list the seeds, just hammering out the details (handling freebies, price, drop date, etc.)

The Jabba's Stash purpling came through, they look great. Did the 2nd Jabba's Cure retain the peppery mango smell, or did the fermenting aspect mature and change the nose?
 
Schwaggy, when you pull the vials to be used from the freezer, do you go immediately to the plants and use it right away? Or do you let it sit and thaw for a while?
I usually take out the vial and immediately wrap it in a dish towel to warm up without condensation on the outside of the baggie (reduces the risk of incidental moisture contact from handling a soggy bag) about an hour before the planned pollination.

I collected and stored some pollen from a male a few months back. After following all of the above steps, and sealing them up, I've tried pollinating a number of different plants with that pollen and have had no success with it. Tried it thawed on a few plants. Tried it right out of the freezer on a different batch of plants. Had a buddy try some on a couple of his plants, still nothing. Is it possibly just a sterile male that was collected from? Is that fairly common?
I would suspect there was some moisture in the container. I still get some vials that will dud because there was some moisture left. Usually, you can see the once fine powder in the vial start to clump up like grated parmesan cheese. I collect a few more vials than I intend to use as insurance against a couple vials clumping.

If you notice the amount of pollen in the vials I posted, it's a relatively small amount. So the day or two without the cap to dry out works well. If you are trying to collect and dry out larger amounts of pollen in a small container, you may need to tip the container on its side to spread out the pollen more (increased surface area = quicker drying). I put vials of larger pollen collections in a small tupperware (lid slightly cracked) with some desiccants to create a very dry micro climate to better dehydrate. Your humidity level is something to consider as well. If you are trying to dry out pollen with a 60% relative humidity in the house, the pollen will struggle to fully dehydrate.

I have not come across a male that puts out sterile pollen. I have experienced reversed female pollen that seems sterile. While it occurs, I don't think it's a common occurrence.

Count me in, as an interested party in your Schwaggy beans. Really like seeing what you are doing. Wishing you all the best.
Thank you :cool:(y)
 

Baja.Beaches

Master Grower
Thank you! I have the okay from a bank to list the seeds, just hammering out the details (handling freebies, price, drop date, etc.)

The Jabba's Stash purpling came through, they look great. Did the 2nd Jabba's Cure retain the peppery mango smell, or did the fermenting aspect mature and change the nose?
The smell on those intensified big time, almost overwhelming at first. Heavily fuel-like fermenting mangoes. I really look forward to trying it, that pheno had lots of vigor, took every thing thrown at it.

I know you were not planning on continuing the CBD line, but I think there would be plenty of interest in it if you did. :D
 
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