PREPARATION OF SILVER THIOSULFATE SOLUTION

J.James

Seed Slingin' Outlaw
Breeder
Silver Thiosulfate (STS) is commonly used to block the action of ethylene in plant cell cultures. Ethylene is a hormone that is present in the gaseous state. Ethylene increases during senescence and ripening, and has been shown to increase in plant cell cultures due to wounding or the presence of auxins. Silver nitrate may be used alone to block the action of ethylene but it is not transported as well as STS thus is seldom used alone.

Prepare a 0.1 M Sodium Thiosulfate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulfate (Product No. S 620) into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate (Product No. S 169) into 100 ml of water. Store the stock solution in the dark until needed to prepare the STS.

The STS solution is prepared with a molar ratio between silver and thiosulfate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of [Ag (S2O3)2] 3-, the active complex for ethylene effect inhibition.

Prepare a 0.02 M STS by slowly pouring 20 ml of 0.1 M silver nitrate stock solution into 80 ml of 0.1 M sodium thiosulfate stock solution. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended.

@Whip Eurass say's
If you're mixing on a counter top, or anything that you care about, cover the work area with aluminum foil before beginning the mix.




 
Last edited:
N
This is straight off the phytotech labs website, I merely copied and pasted it here to make it easier to find as I do with a lot of the information I find floating out in cyberspace.
Nothing wrong with that at all....if anything, these threads bring more info for the general grower on the forum.
 

Bodyne

PICK YOUR OWN
You can buy it reasonable now, and some not that expensive. Twenty20mendo sells some the best price I've seen, if you don't want to make your own.
 

Whip Eurass

Super Active Member
Another thing to remember is to aways cover up your container, you don't want it to get to the roots
If you're mixing on a counter top, or anything that you care about, cover the work area with aluminum foil before beginning the mix. I wish I'd have seen a similar warning.
 

bobjones

New Member
I've always used colloidal silver. Can anyone tell me if STS is worth the extra cost and effort? Is it more effective, or is the benefit that you only have to spray every 3 days rather than twice a day?
 

BrokenHoeFarms

Stress Tester
I've always used colloidal silver. Can anyone tell me if STS is worth the extra cost and effort? Is it more effective, or is the benefit that you only have to spray every 3 days rather than twice a day?
Sounds like you answer your own question to me
(I know nothing about either)
 
D

Deleted member 60

Guest
And it was colloidal silver that did this
DHFHG6OWPBHOVYW67XHM2VP6HE.jpg

....not STS. FYI....the Silver Nitrate component of STS is what they put in us older folks eyes as babies when we wuz born. Now they use an antibiotic for the same purpose. (preventing Pink eye)

Hmmmm..... maybe this "dude" was a chick at one point? Heehee.....

be careful out there folks....:ROFLMAO:
 

Armdog

RDWC GROWER
And it was colloidal silver that did this
View attachment 72550

....not STS. FYI....the Silver Nitrate component of STS is what they put in us older folks eyes as babies when we wuz born. Now they use an antibiotic for the same purpose. (preventing Pink eye)

Hmmmm..... maybe this "dude" was a chick at one point? Heehee.....

be careful out there folks....:ROFLMAO:
LOL
 

Deez. Nutz

Active Member
Yea
I've always used colloidal silver. Can anyone tell me if STS is worth the extra cost and effort? Is it more effective, or is the benefit that you only have to spray every 3 days rather than twice a day?
Yes, it is far better, and far more likely to work. Making the colloidal is easier though. I did it with a piece of 99.9% silver wire and 3, 9 volt batteries.
No need to keep the wire refrigerated or in the dark.
The feminization process is really sped up if you remember to spray just prior to flowering though.
 
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